Beilstein J. Nanotechnol.2016,7, 91–101, doi:10.3762/bjnano.7.11
above detection limits, may be further optimized, and clear the way for nanopore-based sequencing using osmylated DNA.
Keywords: DNA sequencing; α-hemolysin; ion-channelmeasurements; nanopore; osmium tetroxide bipyridine; osmylated oligos; osmylation; single-stranded DNA (ssDNA); Introduction
column, and Figure 3), as well as the absence of unreacted OsBp. The modified oligos were evaluated via the α-HL pore using an instrument custom made for the Chemistry Department of the University of Utah equipped with a glass pore membrane (GNM, see Experimental section). Ionchannelmeasurements were
current and slowed down the translocation of the modified oligo. However the markedly different ion-channelmeasurements observed between dA10dT(OsBp)dA9 and dA10dC(OsBp)dA9 were unexpected (see Graphical Abstract, Figure 4, and Table 2). It turns out that dT(OsBp) yields more relative current obstruction
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Scheme 1:
OsBp with dTMP. Reaction of osmium tetroxide with 2,2’-bipyridine forms a reactive complex (OsBp or...